fixation permeabilization buffer kit (Elabscience Biotechnology)

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Elabscience Biotechnology fixation permeabilization buffer kit
Fixation Permeabilization Buffer Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/intracellular+fixation+permeabilization+buffer+kit/Intracellular+Fixation%2FPermeabilization+Buffer+Kit/pmc13655249-254-10-13
Average 95 stars, based on 72 article reviews

fixation permeabilization buffer kit - by Bioz Stars, 2026-10

95/100 stars

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Staining:

Article Title: Hepatocyte BPGM Induces RET Lactylation and Macrophage Reprogramming to Promote Tumorigenesis in Hepatocellular Carcinoma
Article Snippet: .. Intracellular staining was performed using an Intracellular Fixation/Permeabilization Buffer Kit (E‐CK‐A109, Elabscience, China). .. The single cell suspension was then incubated with Human TruStain FcX (Fc Receptor Blocking Solution, 422301, Biolegend, USA) at room temperature for 10 min, followed by incubation with intracellular antibodies for 30 min at room temperature.

Article Title: Hepatocyte BPGM Induces RET Lactylation and Macrophage Reprogramming to Promote Tumorigenesis in Hepatocellular Carcinoma.
Article Snippet: .. Intracellular staining was performed using an Intracellular Fixation/Permeabilization Buffer Kit (E-CK-A109, Elabscience, China). ..

Article Title: PLA2G16‐Mediated Tetracosatetraenoic Acid Rewires Fatty Acid Oxidation to Impair CD8 + T Cell Immune Function in Promoting Breast Cancer Lung Metastasis
Article Snippet: For measurement of intracellular cytokines production in mouse or human primary CD8 + T cells, cells were first stimulated with Cell Stimulation Cocktail (eBioscience, USA) at 37 °C for 5 h. Cells were harvested, washed twice with DPBS, and conducted with surface staining. .. Then, cells were fixed, permeabilized with Intracellular Fixation/Permeabilization Buffer Kit (Elabscience, China) or Transcription Factor Staining Buffer Set (eBioscience, USA), and stained with antibodies against indicated cytokines at 4 °C for 30 min in the dark. ..

Article Title: Suppression of Nr1d1/Bnip3-dependent mitophagy by notch signaling aggravates inflammatory response in RSV-infected mice lungs.
Article Snippet: 1 Department of Pediatrics, Women and Children’s Hospital, Zhongnan Hospital of Wuhan University, 169# Donghu Road, Wuhan 430062, Hubei, China 2 Jingmen Central Hospital, Central Hospital affiliated to Jingchu University of Technology, Jingmen, China 3 Children’s Digital Health and Data Center, Wuhan University, Wuhan 430062, Hubei, China Abstract Background Alveolar macrophages (AMs) are crucial for defending against respiratory syncytial virus (RSV) and regulating lung inflammation.. Notch signaling is essential for macrophage polarization and its functional diversity.However, the role of macrophages Notch1 in RSV-induced lung injury and its underlying mechanisms remain incompletely understood.. Methods This study used macrophage-specific Notch1 knockout (MKO) mice to investigate the lung lesions and inflammation in RSV-infected mice.

other:

Article Title: Biomimetic sequential immuno-osteogenic regulation via a dual-drug delivery system with PDA coating on sulfonated PEEK promotes bone defect repair
Article Snippet: The following reagents were used in the experiments: PEEK (Nanjing First Plastic Special Engineering Plastic Products Co., Ltd., Nanjing, China), simulated body fluid, dexamethasone, β-glycerol phosphate, ascorbic acid and fluorescein isothiocyanate-labeled (FITC)-labeled phalloidin (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China), 2-methylimidazole (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), Zinc nitrate hexahydrate (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), Recombinant Rat Interleukin-4 (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China), cell counting Kit (CCK)-8, human IL-4R ELISA Kit (YOBIBIO (Shanghai) Biotechnology Co., Ltd., Shanghai, China), Calcein/PI Cell Viability/Cytotoxicity Assay Kit, BCIP/NBT Alkaline Phosphatase Color Development Kit, Alkaline Phosphatase Assay Kit, Alizarin Red S Staining Kit for Osteogenesis and DAPI Staining Solution (Beyotime Biotechnology Co., Ltd., Shanghai, China), Reverse transcription Kit and 2 × RealStar Fast SYBR qPCR Mix (GenStar Biosolutions Co., Ltd., Beijing, China), CD206 Polyclonal antibody (Cat No. 18704-1-AP), iNOS Polyclonal antibody (Cat No. 14142-1-AP), β-actin Polyclonal antibody (Cat No. 20536-1- AP), Osteopontin Polyclonal antibody (Cat No. 22952-1-AP), NF-κB p65 Recombinant antibody (Cat No. 82335-1-RR), Fluorescein (FITC)– conjugated goat anti-rabbit IgG (H + L) (Cat No. SA00003-2), Cy3–conjugated goat anti-rabbit IgG (H + L) (Cat No. SA00009-2) and HRP-conjugated goat anti-rabbit IgG (H + L) (Cat No. SA00001-2) (Proteintech Group, Inc., Wuhan, China), Recombinant Anti-Collagen I antibody (ab138492, Abcam Shanghai Trading Co., Ltd., Shanghai, China), IL-6 Polyclonal Antibody (YP-Ab-16022), IL-10 Polyclonal Antibody (YP-Ab-15986) and IKK alpha Rabbit Polyclonal Antibody (YP-mAb-14162) (UpingBio technology Co., Ltd., Hangzhou China), Bone Morphogenetic Protein 2 (BMP-2) Polyclonal antibody (#AF5163, Affinity Biosciences Group Ltd., Jiangsu, China), APC anti-mouseW CD86 antibody (0.2 μg/μL; #PO3, BioLegend, USA), PE anti-mouse CD206 antibody (0.2 μg/μL; #C068C2, BioLegend, USA), Intracellular Fixation/Permeabilization Buffer Kit (E-CK-A109, Elabscience, China).

Purification:

Article Title: Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer
Article Snippet: Imiquimod (IMQ) was purchased from TCI (Tokyo, Japan). .. Rabbit anti-CD86 polyclonal antibody, rabbit anti-CD206 polyclonal antibody, FITC goat anti-rabbit antibody, IgG/Alexa Fluor555 goat anti-rabbit antibody, APC anti-mouse CD206 antibody, PE anti-mouse CD86 antibody, purified anti-mouse CD16/32 antibody, intracellular Fixation/Permeabilization buffer kit, IL-6 ELISA kit and TNF-α ELISA kit were all purchased from Elabscience Biotechnology (Wuhan, China). .. IL-4 was purchased from Biyuntian Biotechnology (Shanghai, China).

Enzyme-linked Immunosorbent Assay:

Cell Stimulation:

Article Title: In depth study on the mechanism of Xiao-Chai-Hu-Tang in ameliorating irinotecan-induced intestinal toxicity and enhancing antitumor immunity via intestinal microbiota and fecal metabolomics.
Article Snippet: Irinotecan (CPT− 11) is a cornerstone chemotherapeutic for advanced colorectal cancer (CRC), but its clinical use is limited by dose-dependent gastrointestinal (GI) toxicity.. Xiao-Chai-Hu-Tang (XCHT), a classical traditional Chinese medicine formula, alleviates CPT− 11-induced adverse effects, yet its mechanism remains unclear.. This study established a CPT− 11-induced intestinal toxicity model in CRC rats, using multi-omics analyses (16S rRNA sequencing, fecal metabolomics, SCFA quantification) and an antibiotic depletion model to explore XCHT’s mechanism via the gut microbiota-metabolite axis.

Article Title: Recombinant Human MG53 Membrane Repair Protein Enables Improved Cryopreservation Efficiency of Natural Killer Cells.
Article Snippet: Efficient cryopreservation of Natural Killer (NK) cells is fundamental to manufacturing NK cell-based immunotherapy products, but it remains challenging due to irreversible cryoinjuries to cell structures (e.g., the membrane system).. Herein, inspired by the damage repair mechanism of natural organisms, recombinant human MG53 (rhMG53) protein, known as a membrane repair protein, is reported as a functional additive for improving the NK cell cryopreservation efficacy.. The results showed that incubating NK cells with 30 μg/mL rhMG53 protein could preserve the integrity of membranes subjected to mechanical injury, reactive oxygen species damage, and dimethyl sulfoxide (DMSO)-induced toxic injury.

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